Logo JG-Universität MainzProf. Dr. Axel Müller

    

415. Majewski, A.P.; Schallon, A.; Jérôme, V.; Freitag, R.; Müller, A.H.E.; Schmalz, H.: Dual-Responsive Magnetic Core-Shell Nanoparticles for Nonviral Gene Delivery and Cell Separation, Biomacromolecules 13(3), 857 (2012) -- DOI: 10.1021/bm2017756
Abstract:

We present the synthesis of dual-responsive (pH and temperature) magnetic core-shell nanoparticles utilizing the grafting-from approach. First, oleic acid stabilized superparamagnetic maghemite (γ-Fe2O3 nanoparticles (NP’s), prepared by thermal decomposition of iron pentacarbonyl, were surface-functionalized with ATRP initiating sites bearing a dopamine anchor group via ligand exchange. Subsequently, 2-(dimethylamino)ethyl methacrylate (DMAEMA) was polymerized from the surface by ATRP, yielding dual-responsive magnetic core-shell NP’s (γ-Fe2O3@PDMAEMA). The attachment of the dopamine anchor group on the nanoparticles´ surface is shown to be reversible to a certain extent, resulting in a grafting density of 0.15 chains per nm² after purification. Nevertheless, the grafted NP’s show excellent long-term stability in water over a wide pH range, and exhibit a pH- and temperature-dependent reversible agglomeration as revealed by turbidimetry. The efficiency of γ-Fe2O3@PDMAEMA hybrid nanoparticles as a potential transfection agent was explored under standard conditions in CHO-K1 cells. Remarkably, γ-Fe2O3@PDMAEMA led to a twofold increase of the transfection efficiency without increasing the cytotoxicity as compared to polyethyleneimine (PEI) and yielded on average more than 50% transfected cells. Moreover, after transfection with the hybrid nanoparticles the cells acquired magnetic properties that could be used for selective isolation of transfected cells.

Zu dieser Publikation gibt es weitere Dateien zum Download

Passwort

powered by php + PostgreSQL - Letzte Änderung 07.04.2012- Impressum