Logo JG-Universität MainzProf. Dr. Axel Müller


516. Raup, A.; Wang, H.; Synatschke, C.V.; Jérôme, V.; Agarwal, S.; Pergushov, D.V.; Müller, A.H.E.; Freitag, R.: Compaction and transmembrane delivery of pDNA: differences between l-PEI and two types of amphiphilicblock copolymers, Biomacromolecules 18, 808 (2017) -- DOI: 10.1021/acs.biomac.6b01678
Polycations are popular agents for non-viral delivery of DNA to mammalian cells. Adding hydrophobic, biodegradable, or cell penetrating functions could help to improve their performance, which at present is below that of viral agents. A crucial first step in gene delivery is the complexation of the DNA. The characteristics of these ‘polyplexes’ presumably influence or even determine the subsequent steps of membrane passage, intracellular travelling / DNA release, and nuclear up-take. Herein, polyplexes formed with linear poly(ethylenimine) (l-PEI) are compared to complexes generated with functionalized diblock copolymers. While l-PEI interacts only electrostatically with the DNA, interaction in case of the diblock polymers may be mixed- mode. In certain cases transfection efficiency improved when the polyplexes were formed in hypertonic solution. Moreover, whereas conventional PEI-based polyplexes enter the cells via endocytosis, at least one of the diblock agents seemed to promote entry via transient destabilization of the plasma membrane.

There are additional file downloads belonging to this publication


powered by php + PostgreSQL - last modified 2021-08-27- Impressum